A Structural-informatics Approach for Tracing β-Sheets: Building Pseudo-Cα Traces for β-Strands in Intermediate-resolution Density Maps

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A Structural-informatics Approach for Tracing b-Sheets: Building Pseudo-C Traces for b-Strands in Intermediate-resolution Density Maps

Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA We report the development of two computational methods to assist density map interpretation at intermediate resolutions: sheettracer for building pseudo-C models of b-sheets, and a deconvolution method for enhancing features attributed to major secondary ...

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Cryo-electron microscopy (cryo-EM) has produced density maps of various resolutions. Although α-helices can be detected from density maps at 5-8 Å resolutions, β-strands are challenging to detect at such density maps due to close-spacing of β-strands. The variety of shapes of β-sheets adds the complexity of β-strands detection from density maps. We propose a new approach to model traces of β-st...

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Rapid model building of b-sheets in electron-density maps

Correspondence e-mail: [email protected] A method for rapidly building -sheets into electron-density maps is presented. -Strands are identified as tubes of high density adjacent to and nearly parallel to other tubes of density. The alignment and direction of each strand are identified from the pattern of high density corresponding to carbonyl and C atoms along the strand averaged over all re...

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ژورنال

عنوان ژورنال: Journal of Molecular Biology

سال: 2004

ISSN: 0022-2836

DOI: 10.1016/j.jmb.2004.03.038